With high-quality chemistry and enhanced productivity, the BigDye Terminator v3.1 Cycle Sequencing Kit delivers improved agility and accuracy. Achieve greater uniform peak heights, longer and higher-quality reads, and more rigorous base assignments for heterozygote and mutation detection, all safe in the knowledge that you’re fully supported. As your partner, we’re with you, every step of the way.
Created by experts and refined by global teams, the BigDye Terminator v3.1 cycle sequencing kit offers exceptional versatility and high performance. This is a comprehensive, exhaustive solution, built for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
Improved quality of results and success rates, particularly with challenging templates
The BigDye Terminator v3.1 Cycle Sequencing Kit is expertly designed to meet the demands of the vast majority of contemporary applications, including de novo sequencing and resequencing. Consistently generating data with reliably uniform peak heights and optimized signal balance, the BigDye Terminator kit produces long, high-quality reads, and more accurate base assignments for heterozygote and mutation detection.
The chemistry’s powerful formulation is compatible with a variety of templates, including PCR products, plasmids, and large insert clones, such as fosmids and bacterial artificial chromosomes (BACs). However, its dyes are the same as those found in the v3.0 and v1.0/v2.0 kits respectively, meaning no new software or instrument recalibration is required for data analysis. All that’s left to do is integrate v3.1 into your workflow and rest assured that you’ll reap the rewards of its advanced chemistry profile and ease of use.
The challenges of cycle sequencing call for consumables you can trust to deliver. All BigDye sequencing reagents are rigorously tested twice for quality: first for correct formulation and, finally, for consistent performance on our sequencing systems. And if you should need any assistance? Our expert Applied Biosystems field and telephone support teams are always on hand to advise and answer your questions.
BigDye Terminator v3.1 and v1.1 cycle sequencing kits are completely comprehensive and include all required reagents for sequencing 24, 100, 1,000, 5,000, or 25,000 single stranded (ss) or double-stranded (ds) DNA templates. The reagents in each kit are optimized for use with the 3730xl, 3500, SeqStudio, and Seqstudio Flex genetic analyzers.
96- or 384-well plate, 8-well strips, 12-well strips
96- or 384-well plate, 8-well strips, 12-well strips
For use with
3730xl DNA Analyzer, GeneAmp 9700, Veriti Thermal Cycler, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer. SeqStudio Genetic Analyzer, SeqStudio Flex Genetic Analyzer
3730xl DNA Analyzer, GeneAmp 9700, Veriti Thermal Cycler, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer, SeqStudio Genetic Analyzer, SeqStudio Flex Genetic Analyzer
10 x 20 mL tubes of BigDye Terminator v3.1 Ready Reaction Mix, 4 tubes M13 (-21) Primer, 4 tubes pGEM Control DNA, 1 x 233 mL tube of 5X Sequencing Buffer (may be stored at 4°C)
2 × 20 mL tubes of BigDye Terminator v1.1 Ready Reaction Mix, 2 tubes of M13 (-21) Primer, 2 tubes of pGEM Control DNA, one 28-mL tube of 5X Sequencing Buffer (may be stored at 4°C)
No. of reactions
Stability/storage
